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Cosmo Bio USA mcdb131 medium
Mcdb131 Medium, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcdb131+medium/mcdb153+medium/pmc11883658-109-23-25
Average 90 stars, based on 1 article reviews
mcdb131 medium - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Cell Culture:

Article Title: Genetic and pharmacologic enhancement of SUMO2 conjugation prevents and reverses cognitive impairment and synaptotoxicity in a preclinical model of Alzheimer's disease
Article Snippet: The collected SBT02 was loaded on SDS‐PAGE, and fluorescence was monitored using the ChemiDoc MP Imager (Bio‐Rad) with the 488 nm channel. .. Endothelial hCMEC/D3 immortalized cells (Cat. # SCC066, Millipore) were cultured on Corning BioCoat Gelatin Rectangular Canted Neck Culture Flasks w/Vented Cap (CORNING) in MCDB131 medium (Cosmo Bio) containing 10% fetal bovine serum (FBS; SIGMA), 4 ng/mL aFGF (WAKO), 10 ng/mL EGF (WAKO), 50 μg/mL heparin (Gibco Life Technologies), 1 μg/mL hydrocortisone (WAKO Pure Chemical Industries), and 50 μg/mL Gentamicin (Gibco Life Technologies) at 37°C under humidified 5% CO 2 . hCMEC/D3 were cultured on Transwell (Cat. no. 3470; pore size 0.4 μm; CORNING) inserts using endothelial basal medium 2 (EBM‐2) with an endothelial growth medium 2 (EGM‐2) bullet kit containing 2% FBS, antibiotics, and a mixture of growth factors according to the supplier's instructions (LONZA) at 37°C under humidified 5% CO 2 . ..

Article Title: Establishment of a method for evaluating endothelial cell injury by TNF-α in vitro for clarifying the pathophysiology of virus-associated acute encephalopathy.
Article Snippet: .. Primary HUVECs, which were isolated using 0.25% trypsin/14 mM EDTA (Denka Seiken, Tokyo, Japan), were cultured on Corning BioCoat Gelatin Rectangular Canted Neck Culture Flasks w/Vented Cap (CORNING, Rochester, NY) in MCDB131 medium (Cosmo Bio, Tokyo, Japan) containing 10% fetal bovine serum (FBS; Equitech-Bio, Kerrville, TX), 4 ng/ml aFGF (WAKO Pure Chemical Industries, Ltd., Osaka, Japan), 10 ng/ml EGF (WAKO Pure Chemical Industries, Ltd.), 50 μg/ml heparin (Gibco Life Technologies Inc., Grand Island, NY), 1 μg/ml hydrocortisone (WAKO Pure Chemical Industries, Ltd.), and 50 μg/ml Gentamicin (Nacalai Tesque, Kyoto, Japan) at 37°C under humidified 5% CO2. ..

Pore Size:

Article Title: Genetic and pharmacologic enhancement of SUMO2 conjugation prevents and reverses cognitive impairment and synaptotoxicity in a preclinical model of Alzheimer's disease
Article Snippet: The collected SBT02 was loaded on SDS‐PAGE, and fluorescence was monitored using the ChemiDoc MP Imager (Bio‐Rad) with the 488 nm channel. .. Endothelial hCMEC/D3 immortalized cells (Cat. # SCC066, Millipore) were cultured on Corning BioCoat Gelatin Rectangular Canted Neck Culture Flasks w/Vented Cap (CORNING) in MCDB131 medium (Cosmo Bio) containing 10% fetal bovine serum (FBS; SIGMA), 4 ng/mL aFGF (WAKO), 10 ng/mL EGF (WAKO), 50 μg/mL heparin (Gibco Life Technologies), 1 μg/mL hydrocortisone (WAKO Pure Chemical Industries), and 50 μg/mL Gentamicin (Gibco Life Technologies) at 37°C under humidified 5% CO 2 . hCMEC/D3 were cultured on Transwell (Cat. no. 3470; pore size 0.4 μm; CORNING) inserts using endothelial basal medium 2 (EBM‐2) with an endothelial growth medium 2 (EGM‐2) bullet kit containing 2% FBS, antibiotics, and a mixture of growth factors according to the supplier's instructions (LONZA) at 37°C under humidified 5% CO 2 . ..

Isolation:

Article Title: Establishment of a method for evaluating endothelial cell injury by TNF-α in vitro for clarifying the pathophysiology of virus-associated acute encephalopathy.
Article Snippet: .. Primary HUVECs, which were isolated using 0.25% trypsin/14 mM EDTA (Denka Seiken, Tokyo, Japan), were cultured on Corning BioCoat Gelatin Rectangular Canted Neck Culture Flasks w/Vented Cap (CORNING, Rochester, NY) in MCDB131 medium (Cosmo Bio, Tokyo, Japan) containing 10% fetal bovine serum (FBS; Equitech-Bio, Kerrville, TX), 4 ng/ml aFGF (WAKO Pure Chemical Industries, Ltd., Osaka, Japan), 10 ng/ml EGF (WAKO Pure Chemical Industries, Ltd.), 50 μg/ml heparin (Gibco Life Technologies Inc., Grand Island, NY), 1 μg/ml hydrocortisone (WAKO Pure Chemical Industries, Ltd.), and 50 μg/ml Gentamicin (Nacalai Tesque, Kyoto, Japan) at 37°C under humidified 5% CO2. ..



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